Antibody discovery projects are often dependent on both specific antibody format requirements (e.g. bispecifics) and on immunization success.
For some targets, immunization can turn out to be inefficient, in particular for highly conserved targets. In these cases, library-based approaches might be better suited, but these cannot be applied using classical technologies. To overcome format related developability issues it may be necessary to design a library of a large number of variants and test them for e.g. expression level and/or functionality.
AbCheck’s new single copy integration site technology is a first-in-class mammalian cell-based library secretion system which was specifically developed to bridge the gap between library-based technologies and functional sorting: Combining it with our microfluidics technology enables antibody format and immunization-independent use of superior screening capabilities, creating a unique combination of library-based approaches and high throughput functional sorting.
Overview
- Rapid single copy site-specific, irreversible gene insertion of fragments (up to 20kb)
- Enables Microfluidics-based projects independent of immunization success
- Broad applicability with essentially no limitations to antibody formats
- Creation of mammalian cell-based expression libraries for subsequent functional analysis with our microfluidics technology
Key
Advantages
- Controlled copy number insertion – one copy per cell
- High integration efficiency (>90% with drug selection)
- Increased PCR/cloning recovery rate
- Insertion into well-characterized safe-harbor locus ensuring consistent medium-high protein expression across constructs
- Stable cell line with stable gene integration
TECHNOLOGY DIAGRAM
Combining the Advantages of Leading Technologies
Single Copy Integration Site Technology bridges library-based approaches with Microfluidics
